2013 recruits Ben McEvoy, Ben Ross and Billy Hartung are set to play their first game in the brown and gold along with New Zealand rookie Kurt Heatherley.
Matt Suckling and Alex Woodward look set to make their return since suffering knee injuries during the pre-season last year.
Tickets are still on sale for the match, simply head to ticketmaster.com.au to purchase.
Hawthorn v Brisbane Lions
NAB Challenge Round 1
Thursday 13 February
7.10pm, Etihad Stadium
Hawthorn Squad:
2. Jarryd Roughead
3. Jordan Lewis
4. Matt Suckling
5. Sam Mitchell
7. Ben McEvoy
8. Taylor Duryea
9. Shaun Burgoyne
10. Bradley Hill
13. Kyle Cheney
16. Isaac Smith
18. Jonathon Ceglar
19. Jack Gunston
20. David Hale
21. Ben Ross
22. Luke Breust
23. Tim O’Brien
26. Liam Shiels
28. Paul Puopolo
29. Will Langford
30. Luke Lowden
31. Angus Litherland
32. Jonathan Simpkin
33. Cyril Rioli
35. Sam Grimley
36. Derick Wanganeen
38. Mitch Hallahan
39. Alex Woodward
40. Billy Hartung
46. Kurt Heatherley
Brisbane Lions squad:
2. Ryan Harwood
4. James Aish
5. Jack Crisp
6. Josh Green
8. Rohan Bewick
13. Trent West
15. Dayne Zorko
18. Nicholas Robertson
19. Jordan Lisle
22. Marco Paparone
25. Daniel McStay
26. Tom Cutle
29. Andrew Raines
31. James Polkinghorne
32. Sam Mayes
33. Michael Close
34. Jonathan Freeman
35. Ryan Lester
36. Matt Maguire
37. Patrick Wearden
38. Tom Rockliff
40. Isaac Conway
41. Mitch Golby
42. Justin Clarke
43. Nicholas Hayes
44. Archie Smith
45. Jordon Bourke
46. Sam Michael
47. Zachary O'Brien
