Hawthorn is set to be without youngster Lachlan Bramble for Round 1 after the 23-year-old injured his foot during the club's practice game against Collingwood on Friday.
Scans this week confirmed that the young Hawk had a stress fracture in his foot.
Jai Serong and Daniel Howe will also be unavailable to play in Hawthorn's practice match against Richmond on Saturday after they both suffered a concussion during last week's game.
Meanwhile, Luke Breust is expected to make a return on the weekend after progressing through the concussion protocols.
Chad Wingard is also set to be available for the match the following a short stint on the sidelines due to an ankle injury.
Jarman Impey continues his recovery, but is unlikely to be ready for Round 1, while Changkuoth Jiath returned to game play this week at training as he looks to make a return early in the season.
Hawthorn will take on the Tigers at Devonport Oval on Saturday at 1.10pm.
Watch the full AIA Injury Update below for more details on Bramble, Jiath and Impey.
Hawthorn's injury list:
Lachlan Bramble - foot
Daniel Howe - concussion
Jai Serong – concussion
Will Day - ankle
Changkuoth Jiath - PCL
Jarman Impey - ankle/foot